Control:Article Title: AARS1-mediated lactylation of STAT1 drives immune evasion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LDHA Proteintech Cat#19987-1-AP; RRID:AB_10646429 anti-AARS1 Proteintech Cat#17394-1-AP; RRID:AB_2219748 anti-CXCL10 Proteintech Cat#10937-1-AP; RRID:AB_2088002 anti-Tubulin Proteintech Cat#66031-1-Ig; RRID:AB_11042766 anti-Flag-HRP Proteintech Cat#HRP-66008; RRID:AB_2883835 anti-HA-HRP Proteintech Cat#HRP-81290; RRID:AB_3086578 HRP-conjugated Goat Anti-Rabbit IgG (H + L) Proteintech CatSA00001-2; RRID:AB_2722564 HRP-conjugated Goat Anti-Mouse IgG (H + L) Proteintech Cat#SA00001-1; RRID:AB_2722565 anti-STAT1 HuaBio Cat#ET1606-39; RRID:AB_3069734 anti-JAK2 HuaBio Cat#ET1607-35; RRID:AB_3069760 anti-5mC HuaBio Cat#HA601350; RRID:AB_3740270 anti-p-STAT1 ABclonal Cat#AP0135; RRID:AB_2771563 anti-H3K27me3 ABclonal Cat#A2362; RRID:AB_2764322 anti-Pan-Kla PTM Biolab Cat#PTM-1401; RRID:AB_2868521 anti-Myc-HRP GNI Cat#GNI4310; RRID:AB_2885081 anti-CD8 Cell Signaling Technology Cat#98941; RRID:AB_2756376 anti-STAT1-K193la GL Biochem Ltd N/A PE/Cyanine7 anti-mouse CD45 Biolegend Cat#103114; RRID:AB_312979 FITC anti-mouse CD3 Biolegend Cat#100204; RRID:AB_312661 PE anti-mouse CD8 Biolegend Cat#126607; RRID:AB_961300 APC anti-mouse IFN-γ Biolegend Cat#505810; RRID:AB_315404 APC anti-mouse TNF-α Biolegend Cat#506308; RRID:AB_315429 APC anti-human/mouse Granzyme B Biolegend Cat#372204; RRID:AB_2687028 InVivoMAb anti-mouse PD-1 BioXCell Cat#BE0146; RRID:AB_10949053 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089; RRID:AB_1107769 Bacterial and virus strains Trelief 5α Tsingke Cat#DLC101 Stbl3 NCM Biotech Cat#MC102 Biological samples Patient-derived CRC tissue samples Tongji Hospital, Shanghai N/A Chemicals, peptides, and recombinant proteins Lactate Sigma-Aldrich Cat#L1750 Sodium lactate Sigma-Aldrich Cat#71718 Sodium oxamate Target Mol Cat#T19831 Stiripentol Target Mol Cat#T4498 IL-2 Protein, Mouse Target Mol Cat#TMPY-02788 Human IFN-γ MCE Cat#HY-P7025 Mouse IFN-γ MCE Cat#HY-P7071 Fedratinib MCE Cat#HY-10409 Mouse CXCL10 MCE Cat#HY-P7227 IL-2 Protein, Human MCE Cat#HY-P7037 K193-pe Guoping Pharmaceutic Inc N/A K193R-pe Guoping Pharmaceutic Inc N/A Rhodamine B-K193-pe GL Biochem Ltd N/A (Continued on next page) Cell Reports 45, 117094, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rhodamine B-K193-pe GL Biochem Ltd N/A Anti-Flag affinity gels Beyotime Biotechnology Cat#P2271 Anti-HA affinity gels Beyotime Biotechnology Cat#P2287 Protein A + G agarose gels Beyotime Biotechnology Cat#P2197 Collagenase IV Diamond Cat#A004186 Collagenase I Diamond Cat#A004194 Hyaluronidase BBI CO., LTD Cat#B618252 Percoll Cytiva Cat#17089102 2 × Phanta Flash Master Mix Vazyme Cat#P520 Critical commercial assays EZ-press RNA Purification Kit EZBioscience Cat#B0004D EasySepTM Mouse CD8a Positive Selection Kit II Stem Cell Technology Cat#18953 EasySepTM Human CD8+ T cell Isolation Kit Stem Cell Technology Cat#17953 ImmunoCultTM Mouse T cell Activator Kit Stem Cell Technology Cat#100-1572 ImmunoCultTM Human CD3/CD28/CD2 T cell Activator Kit Stem Cell Technology Cat#100-0785 Mouse MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-M3077 Human MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-H6062 Mouse IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-M0021 Human IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-H0050 Intracellular staining kit eBioscience Cat#2809759 Dual-LumiTM Dual Luciferase Reporter Assay Kit Beyotime Cat#RG088S Experimental models: Cell lines HEK293T NCAC Cat#SCSP-502 AGS NCAC Cat#TCHu232 HCT116 Meilunbio Cat#PWE-HU019 MC38 Generous gift of Dr. Lei Lv N/A B16F10 BaiDi Biotechnology Co., Ltd. Cat#C5031 Experimental models: Organisms/strains C57BL/6J JieSiJie Laboratory Animals N/A Oligonucleotides See Tables S2–S5 N/A N/A Recombinant DNA pCMV-Flag-STAT1-EGFP-Neo MiaoLingPlasmid Cat#P39925 pCMV-Myc-JAK1-Neo MiaoLingPlasmid Cat#P72111 pCMV-Myc-JAK2-Neo MiaoLingPlasmid Cat#P72140 pCMV-HA-AARS1-Neo MiaoLingPlasmid Cat#P64879 pCMV-HA-NAA10-Neo MiaoLingPlasmid Cat#P50690 pSIN-EF2-HA-NAA15 MiaoLingPlasmid Cat#P70852 pCMV-HA-NAA40-Neo MiaoLingPlasmid Cat#P78130 pLV3-IFNGR1-Flag-Puro MiaoLingPlasmid Cat#P64806 pLV3-STAT1-β-Flag-Neo MiaoLingPlasmid Cat#P81042 pLV3-IFNGR1-HA-Puro MiaoLingPlasmid N/A pSTAT1-Luc MiaoLingPlasmid Cat#P45284 pRL-TK MiaoLingPlasmid Cat#P0372 HA-P300 This paper N/A HA-CBP This paper N/A Myc-KAT5 This paper N/A Myc-KAT8 This paper N/A (Continued on next page) 18 Cell Reports 45, 117094, March 24, 2026
Virus:Article Title: AARS1-mediated lactylation of STAT1 drives immune evasion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LDHA Proteintech Cat#19987-1-AP; RRID:AB_10646429 anti-AARS1 Proteintech Cat#17394-1-AP; RRID:AB_2219748 anti-CXCL10 Proteintech Cat#10937-1-AP; RRID:AB_2088002 anti-Tubulin Proteintech Cat#66031-1-Ig; RRID:AB_11042766 anti-Flag-HRP Proteintech Cat#HRP-66008; RRID:AB_2883835 anti-HA-HRP Proteintech Cat#HRP-81290; RRID:AB_3086578 HRP-conjugated Goat Anti-Rabbit IgG (H + L) Proteintech CatSA00001-2; RRID:AB_2722564 HRP-conjugated Goat Anti-Mouse IgG (H + L) Proteintech Cat#SA00001-1; RRID:AB_2722565 anti-STAT1 HuaBio Cat#ET1606-39; RRID:AB_3069734 anti-JAK2 HuaBio Cat#ET1607-35; RRID:AB_3069760 anti-5mC HuaBio Cat#HA601350; RRID:AB_3740270 anti-p-STAT1 ABclonal Cat#AP0135; RRID:AB_2771563 anti-H3K27me3 ABclonal Cat#A2362; RRID:AB_2764322 anti-Pan-Kla PTM Biolab Cat#PTM-1401; RRID:AB_2868521 anti-Myc-HRP GNI Cat#GNI4310; RRID:AB_2885081 anti-CD8 Cell Signaling Technology Cat#98941; RRID:AB_2756376 anti-STAT1-K193la GL Biochem Ltd N/A PE/Cyanine7 anti-mouse CD45 Biolegend Cat#103114; RRID:AB_312979 FITC anti-mouse CD3 Biolegend Cat#100204; RRID:AB_312661 PE anti-mouse CD8 Biolegend Cat#126607; RRID:AB_961300 APC anti-mouse IFN-γ Biolegend Cat#505810; RRID:AB_315404 APC anti-mouse TNF-α Biolegend Cat#506308; RRID:AB_315429 APC anti-human/mouse Granzyme B Biolegend Cat#372204; RRID:AB_2687028 InVivoMAb anti-mouse PD-1 BioXCell Cat#BE0146; RRID:AB_10949053 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089; RRID:AB_1107769 Bacterial and virus strains Trelief 5α Tsingke Cat#DLC101 Stbl3 NCM Biotech Cat#MC102 Biological samples Patient-derived CRC tissue samples Tongji Hospital, Shanghai N/A Chemicals, peptides, and recombinant proteins Lactate Sigma-Aldrich Cat#L1750 Sodium lactate Sigma-Aldrich Cat#71718 Sodium oxamate Target Mol Cat#T19831 Stiripentol Target Mol Cat#T4498 IL-2 Protein, Mouse Target Mol Cat#TMPY-02788 Human IFN-γ MCE Cat#HY-P7025 Mouse IFN-γ MCE Cat#HY-P7071 Fedratinib MCE Cat#HY-10409 Mouse CXCL10 MCE Cat#HY-P7227 IL-2 Protein, Human MCE Cat#HY-P7037 K193-pe Guoping Pharmaceutic Inc N/A K193R-pe Guoping Pharmaceutic Inc N/A Rhodamine B-K193-pe GL Biochem Ltd N/A (Continued on next page) Cell Reports 45, 117094, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rhodamine B-K193-pe GL Biochem Ltd N/A Anti-Flag affinity gels Beyotime Biotechnology Cat#P2271 Anti-HA affinity gels Beyotime Biotechnology Cat#P2287 Protein A + G agarose gels Beyotime Biotechnology Cat#P2197 Collagenase IV Diamond Cat#A004186 Collagenase I Diamond Cat#A004194 Hyaluronidase BBI CO., LTD Cat#B618252 Percoll Cytiva Cat#17089102 2 × Phanta Flash Master Mix Vazyme Cat#P520 Critical commercial assays EZ-press RNA Purification Kit EZBioscience Cat#B0004D EasySepTM Mouse CD8a Positive Selection Kit II Stem Cell Technology Cat#18953 EasySepTM Human CD8+ T cell Isolation Kit Stem Cell Technology Cat#17953 ImmunoCultTM Mouse T cell Activator Kit Stem Cell Technology Cat#100-1572 ImmunoCultTM Human CD3/CD28/CD2 T cell Activator Kit Stem Cell Technology Cat#100-0785 Mouse MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-M3077 Human MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-H6062 Mouse IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-M0021 Human IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-H0050 Intracellular staining kit eBioscience Cat#2809759 Dual-LumiTM Dual Luciferase Reporter Assay Kit Beyotime Cat#RG088S Experimental models: Cell lines HEK293T NCAC Cat#SCSP-502 AGS NCAC Cat#TCHu232 HCT116 Meilunbio Cat#PWE-HU019 MC38 Generous gift of Dr. Lei Lv N/A B16F10 BaiDi Biotechnology Co., Ltd. Cat#C5031 Experimental models: Organisms/strains C57BL/6J JieSiJie Laboratory Animals N/A Oligonucleotides See Tables S2–S5 N/A N/A Recombinant DNA pCMV-Flag-STAT1-EGFP-Neo MiaoLingPlasmid Cat#P39925 pCMV-Myc-JAK1-Neo MiaoLingPlasmid Cat#P72111 pCMV-Myc-JAK2-Neo MiaoLingPlasmid Cat#P72140 pCMV-HA-AARS1-Neo MiaoLingPlasmid Cat#P64879 pCMV-HA-NAA10-Neo MiaoLingPlasmid Cat#P50690 pSIN-EF2-HA-NAA15 MiaoLingPlasmid Cat#P70852 pCMV-HA-NAA40-Neo MiaoLingPlasmid Cat#P78130 pLV3-IFNGR1-Flag-Puro MiaoLingPlasmid Cat#P64806 pLV3-STAT1-β-Flag-Neo MiaoLingPlasmid Cat#P81042 pLV3-IFNGR1-HA-Puro MiaoLingPlasmid N/A pSTAT1-Luc MiaoLingPlasmid Cat#P45284 pRL-TK MiaoLingPlasmid Cat#P0372 HA-P300 This paper N/A HA-CBP This paper N/A Myc-KAT5 This paper N/A Myc-KAT8 This paper N/A (Continued on next page) 18 Cell Reports 45, 117094, March 24, 2026
Recombinant:Article Title: AARS1-mediated lactylation of STAT1 drives immune evasion.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-LDHA Proteintech Cat#19987-1-AP; RRID:AB_10646429 anti-AARS1 Proteintech Cat#17394-1-AP; RRID:AB_2219748 anti-CXCL10 Proteintech Cat#10937-1-AP; RRID:AB_2088002 anti-Tubulin Proteintech Cat#66031-1-Ig; RRID:AB_11042766 anti-Flag-HRP Proteintech Cat#HRP-66008; RRID:AB_2883835 anti-HA-HRP Proteintech Cat#HRP-81290; RRID:AB_3086578 HRP-conjugated Goat Anti-Rabbit IgG (H + L) Proteintech CatSA00001-2; RRID:AB_2722564 HRP-conjugated Goat Anti-Mouse IgG (H + L) Proteintech Cat#SA00001-1; RRID:AB_2722565 anti-STAT1 HuaBio Cat#ET1606-39; RRID:AB_3069734 anti-JAK2 HuaBio Cat#ET1607-35; RRID:AB_3069760 anti-5mC HuaBio Cat#HA601350; RRID:AB_3740270 anti-p-STAT1 ABclonal Cat#AP0135; RRID:AB_2771563 anti-H3K27me3 ABclonal Cat#A2362; RRID:AB_2764322 anti-Pan-Kla PTM Biolab Cat#PTM-1401; RRID:AB_2868521 anti-Myc-HRP GNI Cat#GNI4310; RRID:AB_2885081 anti-CD8 Cell Signaling Technology Cat#98941; RRID:AB_2756376 anti-STAT1-K193la GL Biochem Ltd N/A PE/Cyanine7 anti-mouse CD45 Biolegend Cat#103114; RRID:AB_312979 FITC anti-mouse CD3 Biolegend Cat#100204; RRID:AB_312661 PE anti-mouse CD8 Biolegend Cat#126607; RRID:AB_961300 APC anti-mouse IFN-γ Biolegend Cat#505810; RRID:AB_315404 APC anti-mouse TNF-α Biolegend Cat#506308; RRID:AB_315429 APC anti-human/mouse Granzyme B Biolegend Cat#372204; RRID:AB_2687028 InVivoMAb anti-mouse PD-1 BioXCell Cat#BE0146; RRID:AB_10949053 InVivoMAb rat IgG2a isotype control BioXCell Cat#BE0089; RRID:AB_1107769 Bacterial and virus strains Trelief 5α Tsingke Cat#DLC101 Stbl3 NCM Biotech Cat#MC102 Biological samples Patient-derived CRC tissue samples Tongji Hospital, Shanghai N/A Chemicals, peptides, and recombinant proteins Lactate Sigma-Aldrich Cat#L1750 Sodium lactate Sigma-Aldrich Cat#71718 Sodium oxamate Target Mol Cat#T19831 Stiripentol Target Mol Cat#T4498 IL-2 Protein, Mouse Target Mol Cat#TMPY-02788 Human IFN-γ MCE Cat#HY-P7025 Mouse IFN-γ MCE Cat#HY-P7071 Fedratinib MCE Cat#HY-10409 Mouse CXCL10 MCE Cat#HY-P7227 IL-2 Protein, Human MCE Cat#HY-P7037 K193-pe Guoping Pharmaceutic Inc N/A K193R-pe Guoping Pharmaceutic Inc N/A Rhodamine B-K193-pe GL Biochem Ltd N/A (Continued on next page) Cell Reports 45, 117094, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rhodamine B-K193-pe GL Biochem Ltd N/A Anti-Flag affinity gels Beyotime Biotechnology Cat#P2271 Anti-HA affinity gels Beyotime Biotechnology Cat#P2287 Protein A + G agarose gels Beyotime Biotechnology Cat#P2197 Collagenase IV Diamond Cat#A004186 Collagenase I Diamond Cat#A004194 Hyaluronidase BBI CO., LTD Cat#B618252 Percoll Cytiva Cat#17089102 2 × Phanta Flash Master Mix Vazyme Cat#P520 Critical commercial assays EZ-press RNA Purification Kit EZBioscience Cat#B0004D EasySepTM Mouse CD8a Positive Selection Kit II Stem Cell Technology Cat#18953 EasySepTM Human CD8+ T cell Isolation Kit Stem Cell Technology Cat#17953 ImmunoCultTM Mouse T cell Activator Kit Stem Cell Technology Cat#100-1572 ImmunoCultTM Human CD3/CD28/CD2 T cell Activator Kit Stem Cell Technology Cat#100-0785 Mouse MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-M3077 Human MIγ/CXCL9 ELISA Kit Elabscience Cat#E-EL-H6062 Mouse IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-M0021 Human IP-10/CXCL10 ELISA Kit Elabscience Cat#E-EL-H0050 Intracellular staining kit eBioscience Cat#2809759 Dual-LumiTM Dual Luciferase Reporter Assay Kit Beyotime Cat#RG088S Experimental models: Cell lines HEK293T NCAC Cat#SCSP-502 AGS NCAC Cat#TCHu232 HCT116 Meilunbio Cat#PWE-HU019 MC38 Generous gift of Dr. Lei Lv N/A B16F10 BaiDi Biotechnology Co., Ltd. Cat#C5031 Experimental models: Organisms/strains C57BL/6J JieSiJie Laboratory Animals N/A Oligonucleotides See Tables S2–S5 N/A N/A Recombinant DNA pCMV-Flag-STAT1-EGFP-Neo MiaoLingPlasmid Cat#P39925 pCMV-Myc-JAK1-Neo MiaoLingPlasmid Cat#P72111 pCMV-Myc-JAK2-Neo MiaoLingPlasmid Cat#P72140 pCMV-HA-AARS1-Neo MiaoLingPlasmid Cat#P64879 pCMV-HA-NAA10-Neo MiaoLingPlasmid Cat#P50690 pSIN-EF2-HA-NAA15 MiaoLingPlasmid Cat#P70852 pCMV-HA-NAA40-Neo MiaoLingPlasmid Cat#P78130 pLV3-IFNGR1-Flag-Puro MiaoLingPlasmid Cat#P64806 pLV3-STAT1-β-Flag-Neo MiaoLingPlasmid Cat#P81042 pLV3-IFNGR1-HA-Puro MiaoLingPlasmid N/A pSTAT1-Luc MiaoLingPlasmid Cat#P45284 pRL-TK MiaoLingPlasmid Cat#P0372 HA-P300 This paper N/A HA-CBP This paper N/A Myc-KAT5 This paper N/A Myc-KAT8 This paper N/A (Continued on next page) 18 Cell Reports 45, 117094, March 24, 2026
Microscopy:Article Title: Human umbilical cord plasma derived exosome inhibit the NLRP3 inflammasome and neuro-apoptosis in traumatic spinal cord injury model.
Article Snippet: 1 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 3 Department of Neurology, Tehran Medical Science Branch, Amir Al-Momenin Hospital, Islamic Azad University, Tehran, Iran 4 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran 5 Rayan Stem Cells and Regenerative Medicine Research Center, Ravan Sazeh Company, Tehran, Iran 6 Cellular and Molecular Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran Abstract Spinal cord injury (SCI) causes severe functional impairments and involves both primary mechanical damage and secondary inflammation.. Exosomes from human umbilical cord blood (HUCB) are emerging as promising therapies due to their bioactive components that regulate inflammation and support repair.. Thirty-two male rats were randomly divided into four groups: Group A (laminectomy), Group B (contusion), Group C (contusion + PBS), and Group D (contusion + HUCBexosomes).
Incubation:
Article Title: Label-Free Monitoring of Cancer-associated Fibroblast Activation using NADH Fluorescence Lifetime Imaging
Article Snippet: .. Membranes were blocked for 1 h in PBS-T (0.1% Tween-20) and 3% BSA (Sigma Aldrich, Cat. No. A3294-10G), followed by incubation overnight at 4 °C with primary anti-α-SMA antibody (1:1000) (Merck, Cat. No. A5228-200UL) and anti-tubulin antibody (1:1000) (Proteintech, Cat. No. 11224-1-AP)). .. After washing with PBS-T, membranes were incubated for 2h at room temperature with ALEXA488- (Proteintech Cat. No. SA00013-2) and ALEXA647-conjugated secondary (ThermoFisher Scientific, Cat. No. A21235) antibodies and was visualized using an iBRIGHT imager (ThermoFisher Scientific).
Article Title: Xiao-Yao-San suppresses psychological distress-related colorectal cancer metastasis and enhances antitumor immune responses through regulating gut microbiota-bile acid axis.
Article Snippet: For hematoxylin and eosin (H&E) staining, subsequent to deparaffinization and rehydration, sections were stained using the H&E Staining Kit (Beyotime). .. For immunohistochemistry (IHC), sections were processed by standard dewaxing, rehydration, endogenous peroxidase blocking, and antigen retrieval, followed by incubation with primary antibodies: Ki-67 (1:1000, Abcam, Cat. No 15,580), programmed cell death protein-1 (PD1) (1:2000, Proteintech, Cat. No 66,220–1-Ig), lymphocyte-activation gene 3 (LAG-3) (1:2000, Proteintech, Cat. No 80,867–1-RR), and Granzyme B (GZMB) (1:1000, Proteintech, Cat. No 13,588–1-AP). ..
Immunohistochemistry:Article Title: Xiao-Yao-San suppresses psychological distress-related colorectal cancer metastasis and enhances antitumor immune responses through regulating gut microbiota-bile acid axis.
Article Snippet: For hematoxylin and eosin (H&E) staining, subsequent to deparaffinization and rehydration, sections were stained using the H&E Staining Kit (Beyotime). .. For immunohistochemistry (IHC), sections were processed by standard dewaxing, rehydration, endogenous peroxidase blocking, and antigen retrieval, followed by incubation with primary antibodies: Ki-67 (1:1000, Abcam, Cat. No 15,580), programmed cell death protein-1 (PD1) (1:2000, Proteintech, Cat. No 66,220–1-Ig), lymphocyte-activation gene 3 (LAG-3) (1:2000, Proteintech, Cat. No 80,867–1-RR), and Granzyme B (GZMB) (1:1000, Proteintech, Cat. No 13,588–1-AP). ..
Blocking Assay:Article Title: Xiao-Yao-San suppresses psychological distress-related colorectal cancer metastasis and enhances antitumor immune responses through regulating gut microbiota-bile acid axis.
Article Snippet: For hematoxylin and eosin (H&E) staining, subsequent to deparaffinization and rehydration, sections were stained using the H&E Staining Kit (Beyotime). .. For immunohistochemistry (IHC), sections were processed by standard dewaxing, rehydration, endogenous peroxidase blocking, and antigen retrieval, followed by incubation with primary antibodies: Ki-67 (1:1000, Abcam, Cat. No 15,580), programmed cell death protein-1 (PD1) (1:2000, Proteintech, Cat. No 66,220–1-Ig), lymphocyte-activation gene 3 (LAG-3) (1:2000, Proteintech, Cat. No 80,867–1-RR), and Granzyme B (GZMB) (1:1000, Proteintech, Cat. No 13,588–1-AP). ..
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